pbpH [2019-06-24 10:50:56]

class B penicillin-binding protein H, required for [SW|cell wall synthesis ]during cell elongation

locus: BSU13980

pI: 8.89

mw: 76.98 kDa

protein length: 685 aa

gene length: 2058 bp

function: formation of a rod-shaped peptidoglycan cell wall

product: penicillin-binding protein H

essential: no

synonyms: ykuA

Genomic Context

categories

• [category|SW 1|Cellular processes] → [category|SW 1.1|Cell envelope and cell division] → [category|SW 1.1.1|Cell wall synthesis] → [category|SW 1.1.1.7|Penicillin-binding proteins]
• [category|SW 6|Groups of genes] → [category|SW 6.2|Membrane proteins]

Gene

Coordinates: 1,467,805 1,469,862

The protein

Catalyzed reaction/ biological activity

• required for [SW|cell wall synthesis] during cell elongation [Pubmed|12896990]
• driver of [protein|A4C8719E06F774A6EB4D79757CC79CF89E453A54|MreB] dynamics [Pubmed|21636744,21636745]

Protein family

• transpeptidase family (according to Swiss-Prot)

Paralogous protein(s)

• [protein|3B4F035535D6504405567E7C44E72902A11F7447|PbpA]

Structure

• [PDB|2WAF] (Pbp2B from Streptococcus pneumoniae, 31% identity) [pubmed|19233207]

[SW|Localization]

• during vegetative growth: evenly distributed along the membrane [pubmed|14731276]
• localization depends on the availability of peptidoglyan precursors (lipid II) [Pubmed|23895585]

Expression and Regulation

Operons

• genes: [gene|883DFF72888D280A102E53CC1D69A8B1C7BE2907|pbpH]
  description: [pubmed|22383849]

  regulatory mechanism

  • [protein|E5110D9C36E8C55AFD1419987B14A53232165F20|AbrB]: repression, [Pubmed|20817675], in [regulon|E5110D9C36E8C55AFD1419987B14A53232165F20|AbrB regulon]

Biological materials

Mutant

• MGNA-B335 (ykuA::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/1334 NBRP B. subtilis, Japan]
• BKE13980 ([gene|883DFF72888D280A102E53CC1D69A8B1C7BE2907|pbpH]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE13980 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATTCTGATTGCTCTGTTTC, downstream forward: _UP4_TAAAAAACAGGGTGCACAAC
• BKK13980 ([gene|883DFF72888D280A102E53CC1D69A8B1C7BE2907|pbpH]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK13980 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATTCTGATTGCTCTGTTTC, downstream forward: _UP4_TAAAAAACAGGGTGCACAAC

GFP fusion

• 3140 [gene|0467447B10EE7C1FDF86022AB6FBDBABFD32A9E5|trpC]2 [gene|883DFF72888D280A102E53CC1D69A8B1C7BE2907|pbpH]::pSG5058 (cat P_xyl-gfpa-[gene|883DFF72888D280A102E53CC1D69A8B1C7BE2907|pbpH]1-827) [pubmed|14731276], available in [SW|Dirk Jan Scheffers]' lab and in the [http://bgsc.org BGSC]

References

• 12896990,20525796,21636744,21636745,20817675,23199363,23895585,14731276,19233207